DNA ligase

DNA ligase
Enzyme involved in DNA replication. The DNA ligase of E. coliseals nicks in one strand of double-stranded DNA, a reaction required for linking precursor fragments during discontinuous synthesis on the lagging strand. Nicks are breaks in the phosphodiester linkage that leave a free 3&’-OH and 5&’-phosphate. The ligase from phage T4 has the additional property of joining two DNA molecules having completely base-paired ends. DNA ligases are crucial in joining DNA molecules and preparing radioactive probes (by nick translation) in recombinant DNA technology.

Dictionary of molecular biology. 2004.

Игры ⚽ Поможем написать реферат

Look at other dictionaries:

  • DNA ligase — repairing chromosomal damage Identifiers EC number 6.5.1.1 …   Wikipedia

  • DNA-Ligase — Bezeichner Externe IDs CAS Nummer:&# …   Deutsch Wikipedia

  • DNA ligase — ligase ligase n. (Biochem.) An enzyme which catalyzes creation of a covalent bond between two substrates, resulting in a larger product which is a combination of the two starting materials; especially, {DNA ligase}, an enzyme which creates a… …   The Collaborative International Dictionary of English

  • DNA ligase — DNA li·gase (liґgās) an enzyme of the ligase class that catalyzes the energy driven linkage of a double stranded DNA chain with a free 3′ hydroxyl group to one with a 5′ phosphate group, forming a phosphodiester bond between them …   Medical dictionary

  • DNA-Ligase — DNA Ligase,   Molekulargenetik: Enzym, das DNA Fragmente unter Ausbildung einer Esterbindung zwischen Phosphorsäure und Desoxyribose verknüpft. DNA L. spielt v. a. eine Rolle bei der Reparatur von DNA Schäden (DNA Reparatur) sowie bei der… …   Universal-Lexikon

  • DNA ligase — DNR ligazė statusas T sritis chemija apibrėžtis Fermentas, sujungiantis viengrandės DNR fragmentus fosfodiesteriniu ryšiu esant komplementariai DNR matricai. atitikmenys: angl. DNA ligase rus. ДНК лигаза …   Chemijos terminų aiškinamasis žodynas

  • DNA ligase — An enzyme that catalyses a reaction to link two separate DNA molecules via the formation of a phosphodiester bond between the 3 hydroxyl end of one and the 5 phosphate of the other. Its natural role lies in DNA repair and replication. An… …   Glossary of Biotechnology

  • DNA ligase — An enzyme that joins two DNA fragments together through the formation of a new phosphodiester bond …   Dictionary of microbiology

  • DNA ligase —    An enzyme that catalyzes nucleotide phisphodiester bond formation …   Forensic science glossary

  • DNA-Replikation — DNA Replikation. Die Doppelhelix wird durch die Helicase und die Topoisomerase geöffnet. Danach setzt die Primase einen Primer und die …   Deutsch Wikipedia

Share the article and excerpts

Direct link
… Do a right-click on the link above
and select “Copy Link”