DNA ligase

DNA ligase
Enzyme involved in DNA replication. The DNA ligase of E. coliseals nicks in one strand of double-stranded DNA, a reaction required for linking precursor fragments during discontinuous synthesis on the lagging strand. Nicks are breaks in the phosphodiester linkage that leave a free 3&’-OH and 5&’-phosphate. The ligase from phage T4 has the additional property of joining two DNA molecules having completely base-paired ends. DNA ligases are crucial in joining DNA molecules and preparing radioactive probes (by nick translation) in recombinant DNA technology.

Dictionary of molecular biology. 2004.

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